GeneTex中国官方网站
  • China

ACAT1抗体 [N1N3]

Anti-ACAT1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102637
Anti-ACAT1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102637
Anti-ACAT1 antibody [N1N3] used in Western Blot (WB). GTX102637
Anti-ACAT1 antibody [N1N3] used in Western Blot (WB). GTX102637
Anti-ACAT1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX102637
Anti-ACAT1 antibody [N1N3] used in Western Blot (WB). GTX102637

目录号 GTX102637

文献引用 6
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Zebrafish
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 ACAT1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

45 kDa. ( Note )

阳性对照

Mouse brain , 293T , A431 , Molt-4

预测种属反应

Rat, Bovine, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human ACAT1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1949561

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

acetyl-CoA acetyltransferase 1 , ACAT , MAT , T2 , THIL

细胞定位

Mitochondrion

功能与背景

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq]

数据库

  • Gene ID: 38 ACAT1
  • UniProt: P24752 ACAT1

研究领域

產品图片

Anti-ACAT1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102637

GTX102637 ICC/IF Image

ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ACAT1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102637

GTX102637 ICC/IF Image

ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
Anti-ACAT1 antibody [N1N3] used in Western Blot (WB). GTX102637

GTX102637 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX102637 diluted at 1:1000

Anti-ACAT1 antibody [N1N3] used in Western Blot (WB). GTX102637

GTX102637 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:MOLT4(GTX27912)
10% SDS PAGE
GTX102637 diluted at 1:500

Anti-ACAT1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX102637

GTX102637 IHC-P Image

ACAT1 antibody [N1N3] detects ACAT1 protein at cytoplasm on human normal kidney by immunohistochemical analysis.
Sample: Paraffin-embedded human normal kidney.
ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACAT1 antibody [N1N3] used in Western Blot (WB). GTX102637

GTX102637 WB Image

Sample (30 μg of whole cell lysate)
A: whole zebrafish
10% SDS PAGE
GTX102637 diluted at 1:1000

文献引用

Application Reference

Hiromu Yano et al. Cancer Sci 2022; 113 (6) : 2129-2143 Blocking cholesterol efflux mechanism is a potential target for antilymphoma therapy.
实验应用 : WB
Chimalapati S et al. Elife 2020; 9 Vibrio deploys type 2 secreted lipase to esterify cholesterol with host fatty acids and mediate cell egress.
实验应用 : ICC/IF
种属反应 : Human
Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
实验应用 : WB
种属反应 : Human
Marcela de Souza Santos et al. bioRxiv 2019; (Epub) Vibrio deploys Type 2 secreted lipase to esterify cholesterol with host fatty acids and mediate cell egress.
实验应用 : ICC/IF
种属反应 : Human
Yi-Wen Lo et al. Journal of Cellular and Molecular Medicine 2015; 19 (4) : 744-59 mitochondrial proteomics with sirna knockdown to reveal acat1 and mdh2 in the development of *-resistant uterine cancer
实验应用 : WB, ICC/IF
种属反应 : Human
显示更多 +
我要回报文献

产品评论

ACAT1 antibody [N1N3] 目录号 GTX102637

Rating
( Average 4 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-ACAT1 antibody [N1N3] used in Western Blot (WB). GTX102637
Date : Anonymous submitted on 01-Oct-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : F31 cells and F90 cells
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
我要评论产品
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com