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5-HT1A receptor抗体 [N3C1], Internal

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

目录号 GTX104703

文献引用 14

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

46 kDa. ( Note )

预测种属反应

Rabbit, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Synthetic peptide encompassing a sequence within the Intracellular domain of human 5HT1A Receptor. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1241307

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

5-hydroxytryptamine receptor 1A , 5-HT-1A , 5-HT1A , 5HT1a , ADRB2RL1 , ADRBRL1 , G-21 , PFMCD

细胞定位

Cell membrane

数据库

研究领域

產品图片

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-5-HT1A receptor antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104703

GTX104703 ICC/IF Image

5-HT1A receptor antibody [N3C1], Internal detects 5-HT1A receptor protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-5-HT1A receptor antibody [N3C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX104703

GTX104703 IHC-Fr Image

5-HT1A receptor antibody [N3C1], Internal detects 5-HT1A receptor protein by immunohistochemical analysis.
Samples: Frozen Sectioned adult mouse hippocampus.
Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

The data was published in the journal Front Behav Neurosci in 2015.PMID: 25870546

文献引用

Application Reference

Kim HJ et al. Front Cell Neurosci 2023; 17 : 1162966 Possible involvement of microglial P2RY12 and peripheral IL-10 in postpartum depression.
Lim J et al. Front Pharmacol 2022; 13 : 1062650 Differentiated HT22 cells as a novel model for in vitro screening of serotonin reuptake inhibitors.
实验应用 : WB, ICC/IF
种属反应 : Mouse
Saurav Bhowmick et al. eNeuro 2021; 8 (4) Intercellular Adhesion Molecule-1-Induced Posttraumatic Brain Injury Neuropathology in the Prefrontal Cortex and Hippocampus Leads to Sensorimotor Function Deficits and Psychological Stress.
实验应用 : WB
种属反应 : Mouse
2021; : e12771 Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.
Vegas-Su?rez S et al. Br J Pharmacol 2021; 178 (12) : 2516-2532 The effect of 5-HT1A receptor agonists on the entopeduncular nucleus is modified in 6-hydroxydopamine-lesioned rats.
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Application Reference
Kim HJ et al. Front Cell Neurosci 2023; 17:1162966 Possible involvement of microglial P2RY12 and peripheral IL-10 in postpartum depression.
Lim J et al. Front Pharmacol 2022; 13:1062650 Differentiated HT22 cells as a novel model for in vitro screening of serotonin reuptake inhibitors.
实验应用 : WB, ICC/IF
种属反应 : Mouse
Saurav Bhowmick et al. eNeuro 2021; 8 (4) Intercellular Adhesion Molecule-1-Induced Posttraumatic Brain Injury Neuropathology in the Prefrontal Cortex and Hippocampus Leads to Sensorimotor Function Deficits and Psychological Stress.
实验应用 : WB
种属反应 : Mouse
2021;:e12771 Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.
Vegas-Su?rez S et al. Br J Pharmacol 2021; 178 (12):2516-2532 The effect of 5-HT1A receptor agonists on the entopeduncular nucleus is modified in 6-hydroxydopamine-lesioned rats.
Chen L et al. J Int Med Res 2020; 48 (5):3.00061E+14 Electroacupuncture improves synaptic plasticity by regulating the 5-HT1A receptor in hippocampus of rats with chronic unpredictable mild stress.
Kim J et al. Biomol Ther (Seoul) 2020; 28 (3):282-291 Toluene Inhalation Causes Early Anxiety and Delayed Depression with Regulation of Dopamine Turnover, 5-HT1A Receptor, and Adult Neurogenesis in Mice.
Sugimoto K et al. Int J Mol Sci 2020; 22 (1) Serotonin/5-HT1A Signaling in the Neurovascular Unit Regulates Endothelial CLDN5 Expression.
Vegas-Su?rez S et al. Br J Pharmacol 2020; 6-Hydroxydopamine lesion and levodopa treatment modify the effect of buspirone in the substantia nigra pars reticulata.
种属反应 : Rat
Rudyk C et al. J Neuroinflammation 2019; 16 (1):120 Leucine-rich repeat kinase-2 (LRRK2) modulates paraquat-induced inflammatory sickness and stress phenotype.
实验应用 : WB
种属反应 : Mouse
Lim J et al. J Neurosci 2018; (Epub) LRRK2 G2019S induces anxiety/depression-like behavior prior to the onset of motor dysfunction with 5-HT1A receptor upregulation in mice.
Huang F et al. Front Behav Neurosci 2015; 9:70 Deletion of mouse FXR gene disturbs multiple neurotransmitter systems and alters neurobehavior.
Nakamura Y et al. Biotechnol Bioeng 2015; Applications of yeast-based signaling sensor for characterization of antagonist and analysis of site-directed mutants of the human serotonin 1A receptor.
实验应用 : WB
种属反应 : Human
Lin YL et al. Brain Behav Immun 2012; 26 (3):459-68 Prenatal lipopolysaccharide exposure increases anxiety-like behaviors and enhances stress-induced corticosterone responses in adult rats.
实验应用 : WB
种属反应 : Rat
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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